Advances in antibody engineering technology are enabling the designation of various engineered antibody forms for specific purposes in cancer diagnosis and immune therapy. With years of experiences and great deal of researches, scientists from Creative Biolabs have investigated and established a series of approaches for bivalent and bispecific scFv/ Fab construction. The Fab and scFv fragments derived from phage display antibody libraries usually have short half-life and less affinity, whereas in many in vitro and in vivo applications, multivalency of antibody molecules is a desirable property. Linking two or more binding sites efficiently not only increases the functional avidity of antibody molecules, but also make the construction of bi-specific antibodies (antibodies with dual specificities) becoming possible.

    scFv Dimerization (bivalent and bispecific)
We have developed three approaches to generate genetically engineered, dimerized scFv antibody fragments, namely, "Diabody", "Tandem scFv" and "Miniantibody".

http://www.creative-biolabs.com/dimerization-of-scFv-and-fab-fragments.html